Mary Ann Brow has a broad range of experience, both in research and in intellectual property practice, having worked in both large and start-up level corporate settings, and in academia. Ms. Brow’s legal practice addresses clients’ intellectual property needs in a wide range of technology areas, including biotechnology, biochemistry, molecular biology, materials science, and both medical and non-medical devices and processes. She has extensive experience in patent prosecution, litigation, intellectual property due diligence, validity and infringement analyses, as well is in counseling clients regarding intellectual property issues in business development, contracts, and grant funding.
Founding Shareholder, Casimir Jones, S.C., 2007 to present
Associate, Medlen & Carroll, LLP, 2004 to 2007
Patent Agent, Medlen & Carroll, LLP, 2001 to 2004
Director of Intellectual Property and Technology Development, Third Wave Technologies, Inc., 1995-2001
Senior Scientist, Third Wave Technologies, Inc., 1994-2001
Associate Researcher, University of Wisconsin-Madison, 1989-1994
Research Associate, Cetus Corporation, 1986-1989
Research Technician, Research Institute of Scripps Clinic, 1980-1986
University of Wisconsin Law School, J.D., 2004
University of California at Santa Cruz, B.A., Biology, 1980
Hoffmann-LaRoche v. Promega Corp. (N. D. Cal)
Promega Corp. v. Applera Corp. (W.D. Wis.)
Third Wave Technologies, Inc. v. EraGen Corp. (W.D. Wis.)
Third Wave Technologies, Inc. v. Stratagene Corp. (W.D. Wis)
Representative Grants & Awards
Principal Investigator, SBIR Phases I & II, U.S. Dept. of Energy; “Signal Amplification For Specific Nucleic Acid Detection,” 1994-1997
Research Director, NIST Advanced Technology Program grant; “Development of a Generic Technology for the Targeted Detection and Cleavage of DNA and RNA,” 1995-1996
Principal Investigator, SBIR Phase II, National Institutes of Health; “CFLP-Based Determination of Hepatitis C Virus Genotype,” 1997-1999
1. Lyamichev V, M. A. Brow, V. E. Varvel, and J. E. Dahlberg. 1999. Comparison of the 5′ nuclease activities of Taq DNA polymerase and its isolated nuclease domain. Proceedings of the National Academy of Science 96: 6143-6148.
2. Lyamichev, V. L., A. Mast, J. G. Hall, J. R. Prudent, M. W. Kaiser, T. Takova, R. W. Kwiatkowski, T. J. Sander, M. deArruda, D. A. Arco, B. P. Neri and M. A. D. Brow. 1999, Polymorphism identification and quantitative detection of genomic DNA by invasive cleavage of oligonucleotide probes. Nature Biotechnology 17: 292-296.
3. M. A. D. Brow. 1999. The Cleavase I enzyme for mutation and polymorphism scanning. In: PCR Methods Manual. Innis, M. A., et al., eds. Academic Press, Inc. San Diego., pp 537-550.
4. M. A. D. Brow, M. C. Oldenburg, V. Lyamichev, L. M. Heisler, N. Lyamicheva, J. G. Hall, N. J. Eagan, D. M. Olive, L. M. Smith, L. Fors, and J. E. Dahlberg. 1996, Differentiation of bacterial 16S rRNA genes, intergenic regions and Mycobacterium tuberculosis katG genes by structure-specific endonuclease cleavage, Journal of Clinical Microbiology 34: 3129-3137.
5. M. A. D. Brow. 1994. Book review of “The Polymerase Chain Reaction”, K. B. Mullis, F. Ferre and R. A. Gibbs, Eds. Science 265: 817-819.
6. Lyamichev, V. L., M. A. D. Brow and J. E. Dahlberg. 1993. Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases. Science 260:778-783.
7. Lesley, S. A., M. A. D. Brow and Richard R. Burgess. 1991. Use of in vitro protein synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies. Journal of Biological Chemistry 266(4):2632-2638.
8. Johnstone, I. L., P. C. McCabe, P. Greaves, S. J. Gurr, G. E. Cole, M. A. D. Brow, S. E. Unkles, A. J. Clutterbuck, J. R. Kinghorn and M. A. Innis. 1990. Isolation and characterisation of the crnA-niiA-niaD gene cluster for nitrate assimilation in Aspergillus nidulans. Gene 90:181-192.
9. Brow, M. A. D. 1990. Sequencing with Taq DNA polymerase. In: PCR Protocols: A Guide to Methods and Applications. Innis, M. A., et al., eds. Academic Press, Inc. pp189-196.
10. Chiang, Y. L., R. Sheng-Dong, M. A. D. Brow and L. W. Larrick. 1989. Direct cDNA cloning of the rearranged immunoglobulin variable region. BioTechniques 7(4):360-366.
11. Innis, M. A., K. B. Myambo, D. H. Gelfand and M. A. D. Brow. 1988. DNA sequencing with the Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. Proceedings of the National Academy of Science USA 85:9436-9440.
12. Danielson, P., S. Forss-Petter, M. A. D. Brow, L. Calvetta, J. Douglass, R. Milner and J. G. Sutcliffe. 1988. p1B15: A cDNA clone of the rat mRNA encoding cyclophilin. DNA 7(4):261-267.
13. McConlogue, L., M. A. D. Brow and M. A. Innis. 1988. Structure-independent DNA amplification by PCR using 7-deaza-2′-deoxyguanosine. Nucleic Acids Research 16:9869.
14. Lai, C., M. A. D. Brow, K. -A. Nave, A. B. Noronha, R. H. Quarles, F. E. Bloom, R. J. Milner and J. G. Sutcliffe. 1987.Two forms of 1B236/myelin-associated glycoprotein, a cell adhesion molecule for postnatal neural development, are produced by alternative splicing. Proceedings of the National Academy of Science USA 84:4337-4341.
15. McKinnon, R. D., P. Danielson, M. A. D. Brow, F. E. Bloom and J. G. Sutcliffe. 1987. Expression of small cytoplasmic transcripts of the rat identifier element in vivo and in cultured cells. Molecular and Cellular Biology 7:2148-2154.
16. McKinnon, R. D., P. Danielson, M. A. D. Brow, M. Godbout, J. B. Watson and J. G. Sutcliffe. 1987. The neuronal identifier sequence as a positive regulatory element for neuronal gene expression. In: From Message to Mind. Easter, S., et al., eds. Sinauer Associates, Inc. Sunderland, Mass. pp78-89.
17. Forss-Petter, S., R. D. McKinnon, A. -P. Tsou, G. Travis, M. A. D. Brow, M. Kiel, P. Danielson and J. G. Sutcliffe. 1986. Gene expression in the mammalian brain. In: Molecular Genetics in Developmental Neurobiology. Tsukada, Y., ed. Japan Scientific Societies Press. pp227-241.
18. Brow, M. A. D., R. Pesin and J. G. Sutcliffe. 1985. The tetracycline repressor of pSC101. Molecular Biology and Evolution 2(1):1-12.
19. Brow, M. A. D., A. Sen, and J. G. Sutcliffe. 1984. Nucleotide sequence of the transforming gene of m1 murine sarcoma virus. Journal of Virology 49:579-582.
20. Milner, R. J., M. A. D. Brow, D. W. Cleveland, T. M. Shinnick and J. G. Sutcliffe. 1983. Glyceraldehyde 3-phosphate dehydrogenase protein and mRNA are both differentially expressed in adult chickens but not chick embryos. Nucleic Acids Research11:3301-3315.
8,288,093 Detection of nucleic acids by multiple sequential invasive cleavages
8,026,050 Methods for modifying an oligonucleotide
7,829,314 Cleavage of nucleic acids
7,824,862 Methods for analyzing nucleic acid
7,785,852 Cleavage of nucleic acids
7,691,573 Cleavage of nucleic acids
7,601,496 Cleavage of nucleic acids
7,588,891 Invasive cleavage of nucleic acids
7,541,145 Cleavage of nucleic acids
7,514,220 Kits for detection of nucleic acids using invasive cleavage structures and flap endonucleases
7,429,455 Methods for genotyping hepatitis C virus
7,407,782 Nucleic acid detection compositions
7,381,530 Kits for detection of nucleic acids by invasive cleavage
7,354,708 Invasion Assays
7,306,917 Cleavage of nucleic acids
7,282,333 Methods and compositions for nucleic acid analysis
7,273,696 Kits for the detection of target sequences
7,101,672 Target-dependent reactions using structure-bridging oligonucleotides
7,087,381 Detection of target sequences by cleavage of non-target nucleic acids
7,067,643 Systems for the detection of target sequences
7,011,944 Invasive cleavage of nucleic acids
6,913,881 Methods and compositions for detecting target sequences
6,875,572 Nucleic acid detection assays
6,872,816 Nucleic acid detection kits
6,709,819 Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides
6,709,815 Target-dependent reactions using structure-bridging oligonucleotides
6,673,616 Methods and compositions for characterizing nucleic acids
6,458,535 Detection of nucleic acids by multiple sequential invasive cleavages
6,372,424 Rapid detection and identification of pathogens
6,358,691 Target-dependent reactions using structure-bridging oligonucleotides
6,355,437 Target-dependent reactions using structure-bridging oligonucleotides
6,348,314 Invasive cleavage of nucleic acids
6,210,880 Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides
6,194,149 Target-dependent reactions using structure-bridging oligonucleotides
6,090,543 Cleavage of nucleic acids
6,001,567 Detection of nucleic acid sequences by invader-directed cleavage
5,994,069 Detection of nucleic acids by multiple sequential invasive cleavages
5,985,557 Invasive cleavage of nucleic acids
5,888,780 Rapid detection and identification of nucleic acid variants
5,871,911 Method of site-specific nucleic acid cleavage
5,846,717 Detection of nucleic acid sequences by invader-directed cleavage
5,843,654 Rapid detection of mutations in the p53 gene
5,837,450 Detection of target nucleic acid molecules using thermostable 5′ nuclease
5,795,763 Synthesis-deficient thermostable DNA polymerase
5,719,028 Cleavase fragment length polymorphism
5,691,142 Detection of target nucleic acid molecules using synthesis-deficient thermostable DNA polymerase
5,614,402 5′ nucleases derived from thermostable DNA polymerase
5,541,311 Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
5,422,253 Method of site specific nucleic acid cleavage
5,075,216 Methods for DNA sequencing with Thermus aquaticus DNA polymerase